Info

NCBI taxon id: 55311  NCBI; ENA; GoaT
Order: Alismatales
Family: Aponogetonaceae
NCBI lineage: Eukaryota;Viridiplantae;Streptophyta;Embryophyta;Tracheophyta;Spermatophyta;Magnoliopsida;Liliopsida;Aponogetonaceae;Aponogeton;
GoaT genome size (M): unknown (unknown)
GoaT asm span (M): unknown (unknown)
GoaT chr no.: unknown (unknown)
GoaT haploid no.: unknown (unknown)
GoaT ploidy: unknown (unknown)
ToLID prefix:

Specimens

Below is information about specimens collected for this species retrieved from the Sample Tracking System (STS).

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tolid
specimen_id
gal
sex
organism_part
biosample
biospecimen
laApoDist1KDTOL10071ROYAL BOTANIC GARDENS KEWHERMAPHRODITE_MONOECIOUSLEAFSAMEA7522371SAMEA7522292
laApoDist1KDTOL10071ROYAL BOTANIC GARDENS KEWHERMAPHRODITE_MONOECIOUSSTEMSAMEA7522367SAMEA7522292
laApoDist1KDTOL10071ROYAL BOTANIC GARDENS KEWHERMAPHRODITE_MONOECIOUSLEAFSAMEA7522369SAMEA7522292
laApoDist1KDTOL10071ROYAL BOTANIC GARDENS KEWHERMAPHRODITE_MONOECIOUSLEAFSAMEA7522370SAMEA7522292
laApoDist1KDTOL10071ROYAL BOTANIC GARDENS KEWHERMAPHRODITE_MONOECIOUSLEAFSAMEA7522372SAMEA7522292
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Spectra estimates

Below are estimates of genome size, repeat size, heterozygosity based on k-mer specta analysis with GenomeScope2.

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source
specimen
k-mer
k-cov
haploid size
repeat (%)
heterozygosity (%)
model fit (%)
model error (%)
histogram
pacbiolaApoDist13131.124,556,390,48376.170.3494.210.10
histogram.txt
hic-arima2laApoDist1315.14272,451,050,9970.00100.007.330.18
histogram.txt

Sequence data


PacBio run data

Below are stats for each PacBio seqeuncing run collected for this species.

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pipeline
specimen
date
run id
movie
well
tag
yield
N50
sample accession
run accession
barcode
PacBio - HiFilaApoDist12021-02-1879913m64174e_210218_160858A0110203,786,433,33115,320SAMEA7522368
PacBio - HiFi (ULI)laApoDist12023-05-15TRACTION-RUN-588m64178e_230515_223511D01101021,655,277,0108,731SAMEA7522371
PacBio - HiFilaApoDist12023-06-20TRACTION-RUN-645m84047_230620_130731_s3C01203558,467,449,59015,418SAMEA7522371
PacBio - HiFilaApoDist12023-07-07TRACTION-RUN-670m84047_230707_160909_s1C01202224,712,446,56617,468SAMEA7522371
PacBio - HiFilaApoDist12023-09-29TRACTION-RUN-861m84047_230929_150655_s3C01203562,626,116,84615,781SAMEA7522371
PacBio - HiFilaApoDist12023-12-08TRACTION-RUN-992m84093_231208_110757_s1A01200634,872,648,74414,303SAMEA10369677
PacBio - HiFilaApoDist12023-12-09TRACTION-RUN-993m84098_231209_111237_s1A01200287,130,233,53116,274SAMEA10369677
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ONT run data

Below are stats for each ONT seqeuncing run collected for this species.

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pipeline
specimen
date
run id
flowcell
type
yield
N50
sample accession
report
No matching records found

Illumina run data

Below are stats for each Illumina run collected for this species. Click on a row to see associated plots from samtools stats.

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pipeline
specimen
date
run id
read pairs
yield
sample accession
run accession
run status
barcode
Hi-C - Arima v2laApoDist12022-09-1645817_2#16,508,838,724982,834,647,324SAMEA7522371qc completeAponogeton distachyos (1.00)
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Cobionts

Below are results from a screen of the PacBio data using Mash screen against RefSeq assemblies. Only results with identity over 90% are displayed.

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identity
info
0.943372[84 seqs] NZ_BBJS01000082.2 Sphingomonas paucimobilis NBRC 13935 DNA, contig: SP682, whole genome shotgun sequence [...]
0.942757[151 seqs] NZ_JICM01000001.1 Acinetobacter sp. ETR1 contig_1, whole genome shotgun sequence [...]
0.923964[58 seqs] NZ_ATUI01000001.1 Rubrivivax benzoatilyticus JA2 = ATCC BAA-35 strain JA2 F799DRAFT_scaffold00001.1_C, whole genome shotgun sequence [...]
0.920352[30 seqs] NZ_LNIU01000001.1 Rhodococcus equi strain MET contig_10, whole genome shotgun sequence [...]
0.918565[16 seqs] NZ_KB849280.1 Acinetobacter guillouiae CIP 63.46 genomic scaffold acLsj-supercont1.1, whole genome shotgun sequence [...]
0.917512NZ_AP012549.1 Cyanobacterium endosymbiont of Epithemia turgida isolate EtSB Lake Yunoko DNA, complete genome
0.91616[58 seqs] NZ_LZQD01000001.1 Trichormus sp. NMC-1 scaffold1, whole genome shotgun sequence [...]
0.912446NC_018417.1 Candidatus Carsonella ruddii HT isolate Thao2000, complete genome
0.906747[58 seqs] NZ_ATUI01000001.1 Rubrivivax benzoatilyticus JA2 = ATCC BAA-35 strain JA2 F799DRAFT_scaffold00001.1_C, whole genome shotgun sequence [...]
0.905724NZ_CM001438.1 Burkholderiales bacterium JOSHI_001 chromosome, whole genome shotgun sequence
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Species composition by small subunit (SSU) presence in the assembly with MarkerScan.

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specimen
contig
SSU length
attributed taxonomy by SSU
laApoDist1_homcov62atg000004l1806
  • ENA: Eukaryota;Viridiplantae;Streptophyta;Embryophyta;Tracheophyta;Spermatophyta;Magnoliophyta;eudicotyledons;Gunneridae;Pentapetalae;
  • SILVA: Eukaryota;Archaeplastida;Chloroplastida;Charophyta;Phragmoplastophyta;Streptophyta;Embryophyta;Tracheophyta;Spermatophyta;Magnoliophyta;
laApoDist1atg000005l1806
  • ENA: Eukaryota;Viridiplantae;Streptophyta;Embryophyta;Tracheophyta;Spermatophyta;Magnoliophyta;eudicotyledons;Gunneridae;Pentapetalae;
  • SILVA: Eukaryota;Archaeplastida;Chloroplastida;Charophyta;Phragmoplastophyta;Streptophyta;Embryophyta;Tracheophyta;Spermatophyta;Magnoliophyta;
laApoDist1atg000009l1806
  • ENA: Eukaryota;Viridiplantae;Streptophyta;Embryophyta;Tracheophyta;Spermatophyta;Magnoliophyta;eudicotyledons;Gunneridae;Pentapetalae;
  • SILVA: Eukaryota;Archaeplastida;Chloroplastida;Charophyta;Phragmoplastophyta;Streptophyta;Embryophyta;Tracheophyta;Spermatophyta;Magnoliophyta;
laApoDist1atg000020l1807
  • ENA: Eukaryota;Viridiplantae;Streptophyta;Embryophyta;Tracheophyta;Spermatophyta;Magnoliophyta;
  • SILVA: Eukaryota;Archaeplastida;Chloroplastida;Charophyta;Phragmoplastophyta;Streptophyta;Embryophyta;Tracheophyta;Spermatophyta;Magnoliophyta;
laApoDist1_homcov62atg000073l1463
  • ENA: Eukaryota;Viridiplantae;Streptophyta;Embryophyta;Tracheophyta;Spermatophyta;Magnoliophyta;Liliopsida;
  • SILVA: Bacteria;Cyanobacteria;Cyanobacteriia;Chloroplast;
laApoDist1_homcov62atg000075l1802
  • ENA: Eukaryota;Viridiplantae;Streptophyta;Embryophyta;Tracheophyta;Spermatophyta;Magnoliophyta;eudicotyledons;Gunneridae;Pentapetalae;rosids;
  • SILVA: Eukaryota;Archaeplastida;Chloroplastida;Charophyta;Phragmoplastophyta;Streptophyta;Embryophyta;Tracheophyta;Spermatophyta;Magnoliophyta;
laApoDist1_homcov62atg000078l1780
  • ENA: Eukaryota;Viridiplantae;Streptophyta;Embryophyta;Tracheophyta;Spermatophyta;Magnoliophyta;eudicotyledons;Gunneridae;Pentapetalae;rosids;fabids;
  • SILVA: Eukaryota;Archaeplastida;Chloroplastida;Charophyta;Phragmoplastophyta;Streptophyta;Embryophyta;Tracheophyta;Spermatophyta;Magnoliophyta;
laApoDist1atg000079l1806
  • ENA: Eukaryota;Viridiplantae;Streptophyta;Embryophyta;Tracheophyta;Spermatophyta;Magnoliophyta;eudicotyledons;Gunneridae;Pentapetalae;
  • SILVA: Eukaryota;Archaeplastida;Chloroplastida;Charophyta;Phragmoplastophyta;Streptophyta;Embryophyta;Tracheophyta;Spermatophyta;Magnoliophyta;
laApoDist1atg000083l1463
  • ENA: Eukaryota;Viridiplantae;Streptophyta;Embryophyta;Tracheophyta;Spermatophyta;Magnoliophyta;Liliopsida;
  • SILVA: Bacteria;Cyanobacteria;Cyanobacteriia;Chloroplast;
laApoDist1atg000085l1802
  • ENA: Eukaryota;Viridiplantae;Streptophyta;Embryophyta;Tracheophyta;Spermatophyta;Magnoliophyta;eudicotyledons;Gunneridae;Pentapetalae;rosids;
  • SILVA: Eukaryota;Archaeplastida;Chloroplastida;Charophyta;Phragmoplastophyta;Streptophyta;Embryophyta;Tracheophyta;Spermatophyta;Magnoliophyta;
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MarkerScan cobiont assembly by read separation based on observed families (see above). These reads are both aligned to the assembly and independently re-assembled. The quality of these assemblies is assessed by their completeness according to BUSCO, their span and the number of reads they encompass. For more information here.

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specimen
family
classified reads
original assembly
re-assembly
count
(%)
BUSCO
BUSCO
contigs
contig length
number of reads
BUSCO
contigs
contig length
number of reads
No matching records found

Visualisation of a classification of the PacBio reads using a variation autoencoder on the k-mer counts.

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specimen
visualisation
laApoDist1

Canonical tetranucleotide counts for each contig or scaffold reduced to two dimensions with UMAP to allow visualisation.

Features (colours represent quantile bins):

  • Hexamer: Estimated coding density (expected to be higher in microbes than in animals).
  • FastK: The median number of times each 60-mer in the sequence occures across the whole assembly (illustrates repetitiveness)
  • Unique_15mers: Number of unique 15-mers per base pair (illustrates sequence diversity)
  • Is_Connected: Presence of at least one Hi-C connection to another scaffold (absence of connections can indicate contamination)
  • Connections_Base: Number of Hi-C connections per base pair

  • laApoDist1.hifiasm.20240108

Assemblies

In-progress assembly QC.

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specimen
asm
date
contig N50
contigs
scaffold N50
scaffolds
length
BUSCO
merqury
laApoDist1_homcov62hifiasm2024-06-1413,071,1705,8564,821,209,368C:98.8%[S:90.1%,D:8.7%],F:0.7%,M:0.5%,n:425Q59.9-C98.0(HiFi)
laApoDist1hifiasm.purging2024-01-0814,659,3161,9433,679,310,562C:89.6%[S:81.6%,D:8.0%],F:1.9%,M:8.5%,n:425Q62.2-C84.8(HiFi)
laApoDist1hifiasm.scaffolding.yahs2024-01-089,573,9822,523168,211,6811,4923,679,516,762C:89.6%[S:82.1%,D:7.5%],F:1.9%,M:8.5%,n:425Q62.2-C84.8(HiFi)
laApoDist1hifiasm2024-01-0814,014,2915,4384,550,066,198C:98.9%[S:90.4%,D:8.5%],F:0.7%,M:0.4%,n:425Q59.7-C97.8(HiFi)
laApoDist1hifiasm-hic.purging2024-01-089,811,7072,2893,542,808,440C:88.9%[S:79.5%,D:9.4%],F:1.4%,M:9.7%,n:425Q62.4-C83.2(HiFi)
laApoDist1hifiasm-hic.scaffolding.yahs2024-01-087,810,0002,782158,596,1671,5643,543,052,040C:88.9%[S:80.0%,D:8.9%],F:1.4%,M:9.7%,n:425Q62.4-C83.2(HiFi)
laApoDist1hifiasm-hic.hap22024-01-084,697,9791,7821,683,684,816C:48.0%[S:38.1%,D:9.9%],F:3.3%,M:48.7%,n:425-
laApoDist1hifiasm-hic.hap12024-01-088,840,1116,1704,189,296,947C:93.4%[S:83.5%,D:9.9%],F:1.2%,M:5.4%,n:425Q59.7-C97.8(HiFi)
laApoDist1_homcov62hifiasm-hic.hap22024-06-1410,846,0222,7154,587,129,010C:98.6%[S:91.1%,D:7.5%],F:0.7%,M:0.7%,n:425Q63.8-C98.0(HiFi)
laApoDist1_homcov62hifiasm-hic.hap12024-06-1410,466,0975,3934,652,358,525C:98.3%[S:89.4%,D:8.9%],F:0.7%,M:1.0%,n:425Q60.5-C98.0(HiFi)
laApoDist1_homcov62hifiasm-hic.scaffolding_hap1.yahs2024-06-147,839,0006,331223,618,3025,1404,652,596,725C:98.4%[S:89.9%,D:8.5%],F:0.7%,M:0.9%,n:425Q60.5-C98.0(HiFi)
laApoDist1_homcov62hifiasm-hic.scaffolding_hap2.yahs2024-06-147,799,2663,650220,591,7492,5344,587,352,210C:98.9%[S:91.8%,D:7.1%],F:0.7%,M:0.4%,n:425Q63.8-C98.0(HiFi)

Organelles

In-progress organelle results from MitoHiFi or Oatk.

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specimen
asm
organelle
date
length
genes
frameshifts
is circular
seqs
reference
laApoDist1_homcov62oatk.readspltd2024-06-14153,518108rps2True1NC_053914.1; 154,167 bp; 130 genes
laApoDist1_homcov62oatk.readsmito2024-06-14231,09146NoneTrue1NC_088727.1; 256,174 bp; 44 genes
laApoDist1oatk.readspltd2024-01-08153,518108rps2True1NC_053914.1; 154,167 bp; 130 genes
laApoDist1oatk.readsmito2024-01-08231,09149ND1True1OQ948332.1; 876,608 bp; 61 genes