Info

NCBI taxon id: 592556  NCBI; ENA; GoaT
Order: Hemiptera
Family: Cicadellidae
NCBI lineage: Eukaryota;Metazoa;Ecdysozoa;Arthropoda;Hexapoda;Insecta;Pterygota;Neoptera;Paraneoptera;Hemiptera;Auchenorrhyncha;Membracoidea;Cicadellidae;Deltocephalinae;Cicadulini;Mocydia;
GoaT genome size (M): 4,658 (ancestor)
GoaT asm span (M): 1,317 (ancestor)
GoaT chr no.: 21 (ancestor)
GoaT haploid no.: 9 (ancestor)
GoaT ploidy: 3 (ancestor)
ToLID prefix: ihMocCroe

Specimens

Below is information about specimens collected for this species retrieved from the Sample Tracking System (STS).

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tolid
specimen_id
gal
sex
organism_part
biosample
biospecimen
ihMocCroe1Ox000341UNIVERSITY OF OXFORDNOT_COLLECTEDWHOLE_ORGANISMSAMEA8603337SAMEA8563713
ihMocCroe2Ox001057UNIVERSITY OF OXFORDNOT_COLLECTEDWHOLE_ORGANISMSAMEA8603925SAMEA8603269
ihMocCroe3NHMUK014444846NATURAL HISTORY MUSEUMNOT_PROVIDEDABDOMENSAMEA115599728SAMEA115574653
ihMocCroe4NHMUK014444844NATURAL HISTORY MUSEUMNOT_PROVIDEDABDOMENSAMEA115599725SAMEA115574652
ihMocCroe5NHMUK014444861NATURAL HISTORY MUSEUMNOT_PROVIDEDABDOMENSAMEA115599699SAMEA115574639
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Spectra estimates

Below are estimates of genome size, repeat size, heterozygosity based on k-mer specta analysis with GenomeScope2.

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source
specimen
k-mer
k-cov
haploid size
repeat (%)
heterozygosity (%)
model fit (%)
model error (%)
histogram
pacbioihMocCroe33116.832,101,206,93752.841.3397.640.14
histogram.txt
hic-arima2ihMocCroe231180.91,875,544,26365.811.4196.540.24
histogram.txt
pacbioihMocCroe1318.7942,007,981,31953.131.6098.970.18
histogram.txt

Sequence data


PacBio run data

Below are stats for each PacBio seqeuncing run collected for this species.

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pipeline
specimen
date
run id
movie
well
tag
yield
N50
sample accession
run accession
barcode
PacBio - HiFiihMocCroe12021-06-2283503m64230e_210622_180142D01102218,101,901,49617,455SAMEA8603337
PacBio - HiFiihMocCroe12021-07-0284025m64230e_210702_175202A01102219,275,682,61614,453SAMEA8603337
PacBio - HiFiihMocCroe32025-03-14TRACTION-RUN-1818m84098_250314_153233_s3C01208574,013,469,09413,010SAMEA115599728
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ONT run data

Below are stats for each ONT seqeuncing run collected for this species.

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pipeline
specimen
date
run id
flowcell
type
yield
N50
sample accession
report
No matching records found

Illumina run data

Below are stats for each Illumina run collected for this species. Click on a row to see associated plots from samtools stats.

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pipeline
specimen
date
run id
read pairs
yield
sample accession
run accession
run status
barcode
Hi-C - Arima v2ihMocCroe22023-10-1647973_4#16,034,926,774911,273,942,874SAMEA8603925qc complete
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Cobionts

Below are results from a screen of the PacBio data using Mash screen against RefSeq assemblies. Only results with identity over 90% are displayed.

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identity
info
0.989607NZ_CP016223.1 Candidatus Sulcia muelleri strain NC, complete genome
0.97868[43 seqs] NZ_JQAM01000001.1 Wolbachia pipientis strain wRec s1, whole genome shotgun sequence [...]
0.940539[20 seqs] NZ_JRLH01000001.1 Arsenophonus endosymbiont str. Hangzhou of Nilaparvata lugens strain Hangzhou contig1, whole genome shotgun sequence [...]
0.936296NC_018417.1 Candidatus Carsonella ruddii HT isolate Thao2000, complete genome
0.921343[256 seqs] NZ_ACFP01000256.1 Wolbachia endosymbiont of Muscidifurax uniraptor Contig561, whole genome shotgun sequence [...]
0.913038[2 seqs] NC_008513.1 Buchnera aphidicola BCc, complete genome [...]
0.910933[833 seqs] NC_031481.1 Plasmodium gaboni strain SY75 chromosome 1, whole genome shotgun sequence [...]
0.909049[2 seqs] NC_004344.2 Wigglesworthia glossinidia endosymbiont of Glossina brevipalpis DNA, complete genome [...]
0.906067[2 seqs] NC_008513.1 Buchnera aphidicola BCc, complete genome [...]
0.905724NC_009987.1 Spiroplasma kunkelii virus SkV1_CR2-3x, complete genome
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Species composition by small subunit (SSU) presence in the assembly with MarkerScan.

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specimen
contig
SSU length
attributed taxonomy by SSU
No matching records found

MarkerScan cobiont assembly by read separation based on observed families (see above). These reads are both aligned to the assembly and independently re-assembled. The quality of these assemblies is assessed by their completeness according to BUSCO, their span and the number of reads they encompass. For more information here.

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specimen
family
classified reads
original assembly
re-assembly
count
(%)
BUSCO
BUSCO
contigs
contig length
number of reads
BUSCO
contigs
contig length
number of reads
No matching records found

Visualisation of a classification of the PacBio reads using a variation autoencoder on the k-mer counts.

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specimen
visualisation
ihMocCroe1

Canonical tetranucleotide counts for each contig or scaffold reduced to two dimensions with UMAP to allow visualisation.

Features (colours represent quantile bins):

  • Hexamer: Estimated coding density (expected to be higher in microbes than in animals).
  • FastK: The median number of times each 60-mer in the sequence occures across the whole assembly (illustrates repetitiveness)
  • Unique_15mers: Number of unique 15-mers per base pair (illustrates sequence diversity)
  • Is_Connected: Presence of at least one Hi-C connection to another scaffold (absence of connections can indicate contamination)
  • Connections_Base: Number of Hi-C connections per base pair


    Assemblies

    In-progress assembly QC.

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    specimen
    asm
    date
    contig N50
    contigs
    scaffold N50
    scaffolds
    length
    BUSCO
    merqury
    No matching records found

    Organelles

    In-progress organelle results from MitoHiFi or Oatk.

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    specimen
    asm
    organelle
    date
    length
    genes
    frameshifts
    is circular
    seqs
    reference
    No matching records found