Info

NCBI taxon id: 132597  NCBI; ENA; GoaT
Order: Coleoptera
Family: Cerambycidae
NCBI lineage: Eukaryota;Metazoa;Ecdysozoa;Arthropoda;Hexapoda;Insecta;Pterygota;Neoptera;Endopterygota;Coleoptera;Polyphaga;Cucujiformia;Chrysomeloidea;Cerambycidae;Cerambycinae;Clytini;Clytus;
GoaT genome size (M): 528 (ancestor)
GoaT asm span (M): 687 (ancestor)
GoaT chr no.: 20 (direct)
GoaT haploid no.: 10 (direct)
GoaT ploidy: 2 (direct)
ToLID prefix: icClyArie

Specimens

Below is information about specimens collected for this species retrieved from the Sample Tracking System (STS).

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tolid
specimen_id
gal
sex
organism_part
biosample
biospecimen
icClyArie1Ox000566UNIVERSITY OF OXFORDNOT_COLLECTEDTHORAXSAMEA7701431SAMEA7701330
icClyArie1Ox000566UNIVERSITY OF OXFORDNOT_COLLECTEDHEADSAMEA7701431SAMEA7701330
icClyArie1Ox000566UNIVERSITY OF OXFORDNOT_COLLECTEDABDOMENSAMEA7701432SAMEA7701330
icClyArie2NHMUK014036932NATURAL HISTORY MUSEUMNOT_PROVIDEDABDOMENSAMEA112221892SAMEA112221810
icClyArie2NHMUK014036932NATURAL HISTORY MUSEUMNOT_PROVIDEDHEADSAMEA112222118SAMEA112221810
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Spectra estimates

Below are estimates of genome size, repeat size, heterozygosity based on k-mer specta analysis with GenomeScope2.

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source
specimen
k-mer
k-cov
haploid size
repeat (%)
heterozygosity (%)
model fit (%)
model error (%)
histogram
10xicClyArie13119.481,096,070,23453.942.0698.630.37
histogram.txt
pacbioicClyArie13111.211,067,009,33150.201.8698.380.16
histogram.txt
hic-arima2icClyArie13136.631,075,156,9460.00100.002.940.19
histogram.txt

Sequence data


PacBio run data

Below are stats for each PacBio seqeuncing run collected for this species.

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pipeline
specimen
date
run id
movie
well
tag
yield
N50
sample accession
run accession
barcode
PacBio - HiFiicClyArie12021-03-1380510m64125e_210313_151020C01101212,307,081,93211,856SAMEA7701432
PacBio - HiFiicClyArie12021-03-2080717m64178e_210320_161720B01101212,921,478,23411,009SAMEA7701432
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ONT run data

Below are stats for each ONT seqeuncing run collected for this species.

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pipeline
specimen
date
run id
flowcell
type
yield
N50
sample accession
report
No matching records found

Illumina run data

Below are stats for each Illumina run collected for this species. Click on a row to see associated plots from samtools stats.

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pipeline
specimen
date
run id
read pairs
yield
sample accession
run accession
run status
barcode
Chromium genomeicClyArie12021-03-2336958_4#44135,108,03620,401,313,436SAMEA7701432qc completeClytus arietis (1.00)
Chromium genomeicClyArie12021-03-2336958_4#41107,713,34016,264,714,340SAMEA7701432qc completeClytus arietis (1.00)
Chromium genomeicClyArie12021-03-2336958_4#43103,545,74815,635,407,948SAMEA7701432qc completeClytus arietis (1.00)
Chromium genomeicClyArie12021-03-2336958_4#4291,244,87813,777,976,578SAMEA7701432qc completeClytus arietis (1.00)
Hi-C - Arima v2icClyArie12021-04-0737178_4#6687,241,930103,773,531,430SAMEA7701431qc completeClytus arietis (1.00)
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Cobionts

Below are results from a screen of the PacBio data using Mash screen against RefSeq assemblies. Only results with identity over 90% are displayed.

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identity
info
0.956847[157 seqs] NZ_LSYX01000001.1 Wolbachia endosymbiont of Dactylopius coccus strain wDacA Contig_1, whole genome shotgun sequence [...]
0.929005NC_018417.1 Candidatus Carsonella ruddii HT isolate Thao2000, complete genome
0.921098[256 seqs] NZ_ACFP01000256.1 Wolbachia endosymbiont of Muscidifurax uniraptor Contig561, whole genome shotgun sequence [...]
0.906409[2017 seqs] NW_004087753.1 Ichthyophthirius multifiliis unplaced genomic scaffold scaff_1120509249386, whole genome shotgun sequence [...]
0.906067[2 seqs] NC_008513.1 Buchnera aphidicola BCc, complete genome [...]
0.903242NZ_AP013293.1 Candidatus Sulcia muelleri PSPU DNA, complete genome
0.902135[28 seqs] NZ_LTBM01000001.1 Candidatus Phytoplasma oryzae isolate Mbita1 Pmin.contig.0_1, whole genome shotgun sequence [...]
0.900227NC_011047.1 Candidatus Phytoplasma mali strain AT complete chromosome
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Species composition by small subunit (SSU) presence in the assembly with MarkerScan.

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specimen
contig
SSU length
attributed taxonomy by SSU
No matching records found

MarkerScan cobiont assembly by read separation based on observed families (see above). These reads are both aligned to the assembly and independently re-assembled. The quality of these assemblies is assessed by their completeness according to BUSCO, their span and the number of reads they encompass. For more information here.

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specimen
family
classified reads
original assembly
re-assembly
count
(%)
BUSCO
BUSCO
contigs
contig length
number of reads
BUSCO
contigs
contig length
number of reads
No matching records found

Visualisation of a classification of the PacBio reads using a variation autoencoder on the k-mer counts.

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specimen
visualisation
icClyArie1

Canonical tetranucleotide counts for each contig or scaffold reduced to two dimensions with UMAP to allow visualisation.

Features (colours represent quantile bins):

  • Hexamer: Estimated coding density (expected to be higher in microbes than in animals).
  • FastK: The median number of times each 60-mer in the sequence occures across the whole assembly (illustrates repetitiveness)
  • Unique_15mers: Number of unique 15-mers per base pair (illustrates sequence diversity)
  • Is_Connected: Presence of at least one Hi-C connection to another scaffold (absence of connections can indicate contamination)
  • Connections_Base: Number of Hi-C connections per base pair


    Assemblies

    In-progress assembly QC.

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    specimen
    asm
    date
    contig N50
    contigs
    scaffold N50
    scaffolds
    length
    BUSCO
    merqury
    No matching records found

    Organelles

    In-progress organelle results from MitoHiFi or Oatk.

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    specimen
    asm
    organelle
    date
    length
    genes
    frameshifts
    is circular
    reference
    No matching records found